Date: Thu, 18 Dec 1997 18:06:24 GMT Server: Apache/1.0.1 Content-type: text/html Content-length: 2674 Last-modified: Tue, 15 Oct 1996 14:24:59 GMT Neogen Substrates Neogen Corporation Elisa Technologies

READY-TO-USE SUBSTRATES

Neogen Corporation manufactures Ready-To-Use chromogenic substrates for use with horseradish peroxidase and alkaline phosphatase ELISA assays. These one-step substrates are used around the world by commercial immunoassay test kit manufacturers and researchers.


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K-Blue Substrate K-Bluer Substrate (TMB):

K-Blue is a one bottle stabilized chromogenic substrate for horseradish peroxidase based ELISA assays. It contains both 3,3', 5,5' tetramethylbenzidine (TMB) and hydrogen peroxide (H2O2) in a one bottle format with greatly enhanced stability and sensitivity.

K-Blue eliminates the previous mixing of TMB and H2O2 prior to enzyme assay. It is stabilized with proprietary reagents and can be stored at 4øC for fourteen months with no loss of reactivity.

An independent laboratory study found that K-Blue out performed ten other commercially available TMB substrates. Click here to request a copy of the study.


K-Gold™ Substrate (PNPP):

K-Gold is a one-bottle stabilized chromogenic substrate for use with alkaline phosphatase based ELISA assays. K-Gold utilizes the substrate p-nitrophenylphosphate (PNPP) which is reacted upon by alkaline phosphatase to produce the chromophore p-nitrophenol. K-Gold is supplied in a convenient, ready-to-use stabilizing buffer with a pH of 9.3 ñ 0.1.

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Frequently Asked Questions - Ready-To-Use Substrates

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